Flag antibody abcam
WebApr 10, 2024 · (D) Western blot of FLAG indicating AC5, AC6, and AC7 expression in 20 μg protein from HEK293T whole cell lysates separated on 10% SDS-PAGE, probed with mouse monoclonal HRP-anti-FLAG antibody (1:1000, Abcam, Cambridge, MA, USA). HEK293T cells not expressing a FLAG-tagged protein used as antibody control; β-actin as the … WebApr 10, 2024 · Supernatant was incubated with anti-FLAG (SIGMA) or anti-IgG (santa cruz) antibody for 3 hours at 4 °C, and pulled down using Protein G Sepharose beads (GenScript), then washed three times for 15 min in 0.1% digitonin-HEPES buffer, and collected in cell lysis buffer [10% 1M tris-HCl (pH=6.8), 4% SDS, 20% glycerol, 0.01% …
Flag antibody abcam
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WebAntibodies that detect DYKDDDDK Tag can be used in several scientific applications, including Western Blot, Immunocytochemistry, ELISA, Immunoprecipitation and Flow Cytometry. Our DYKDDDDK Tag monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse, Rabbit, Rat, Goat and … WebMonoclonal ANTI-FLAG® M2 antibody produced in mouse 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4) Synonym (s): flag …
WebThis product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and … WebTag-ChIP-IT ® Advantages. Ideal for targets without ChIP antibodies or with low affinity antibodies. Enables studies of isoforms, mutations and truncations. AM-tag design maximizes enrichment efficiency of low abundance proteins. AM-tag has little to no cross-reactivity with mammalian systems for reduced background.
WebANTI-FLAG® High Sensitivity, M2 coated 96-well plates. Synonym (s): Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk. Compare. Product No. WebAnti-FLAG Antibody Products from Abcam. The following lists a number of FLAG antibodies from various suppliers. The target antigen refers to the FLAG epitope tag, …
WebDetection was performed with ANTI-FLAG® M2 monoclonal primary antibody, anti-mouse-HRP secondary antibody, and ECL™ chemiluminescent substrate. Figure 3. Comparative Sensitivity of 3xFLAG®. Each protein fusion tag was cloned separately on the C terminal of GST. The purified proteins were diluted from 1 ug to 0.01 ng and analyzed.
WebProduct name Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies Description Rabbit polyclonal to … how does a horse costWebMonoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse. Synonym (s): Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti … how does a horse get cogginsWebMonoclonal Anti-FLAG ® (rat IgG1 isotype) is derived from the hybridoma 6F7 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with the FLAG ® … how does a horse get colicWebDYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG ® M2 Antibody) Applications: WB, FCM, IP Conjugate/Tag: Inquire Quantity: 100 µl Citations: (455) … how does a horse drawn plow workWebJan 11, 2013 · Antibodies against XBP1 (M-186), CD31, and GAPDH were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA), whereas antibody against FLAG was from Abcam, and anti-LC3 (M186-3) was from MBL. Antibodies against IRE1α, pIRE1α, and BECLIN-1 (Autophagy Kit-4445) were purchased from Cell Signaling. Endostatin … how does a horse bit workWebApr 14, 2024 · For SCFS in presence of a β1 integrin blocking antibody (clone AIIB2, DSHB) or β1 integrin activating antibody (clone 12G10, ab30394, abcam) cells were incubated with a 1:100 (vol/vol) dilution ... phorms neckarsulmWebApr 12, 2024 · The coverslips were incubated with primary antibodies against USP1 (Cell Signaling Technology, Cat# 8033, 1:200) and TAZ (Abcam, ab224239, 1:200) at 4 °C overnight. how does a horse fly bite you